Distinct features of circulating microparticles and their relationship with disease activity in inflammatory bowel disease

Authors Evangelos Voudoukis, Eleni-Kyriaki Vetsika, Konstantina Giannakopoulou, Konstantinos Karmiris, Angeliki Theodoropoulou, Aekaterini Sfridaki, Vassilis Georgoulias, Gregorios A. Paspatis, Ioannis E. Koutroubakis.


Background There is evidence that circulating microparticles (MPs) and annexin (+) platelet-derived MPs (PDMPs) are increased in inflammatory bowel disease (IBD). The aim of our study was to characterize the abundance, origin, and annexin V binding of MPs in patients with IBD and correlate them with the disease characteristics.

Methods Case-control study of 46 IBD patients (23 Crohn's disease, 23 ulcerative colitis) and 40 matched healthy controls (HC). MPs were divided according to annexin V binding, their origin was estimated based on specific cell membrane markers in plasma samples and their number was calculated via flow cytometry. Clinical and laboratory activity indices were also analyzed.

Results Annexin (-) PDMPs (P=0.0004), total (P=0.04) and annexin (+) monocyte-derived MPs (P=0.02) were increased and annexin (-) total MPs (P=0.0007) were decreased in IBD patients compared to HC. The annexin (+)/(-) ratio of all MP types were significantly elevated in IBD patients compared to HC (P<0.003). IBD patients with active disease displayed elevated total and annexin (+) total MPs, total, annexin (+) and (-) PDMPs compared with those in remission (P<0.05). Annexin (-) PDMPs were considerably increased in IBD patients with active compared to those with inactive disease (P=0.0013). Total and annexin (-) PDMPs were significantly correlated with most of the disease activity indices (P<0.05).

Conclusion The majority of circulating MPs, their counterparts and particularly annexin (-) PDMPs are increased in active IBD patients. Annexin (+)/(-) ratio proved to be the most reliable distinctive MP index between HC and IBD patients.

Keywords Microparticles, inflammatory bowel disease, Crohn's disease, ulcerative colitis

Ann Gastroenterol 2016; 29 (2): 180-187

DOI: http://dx.doi.org/10.20524/aog.2016.0010

Original Articles